Hey all,

I viewed the videos in detail and will provide comments in person tomorrow. (Amanda, can we do 1:00 PM instead of 11:00 AM?).

I am working on the first draft of the first video, editing it down while making the audio file, which I’ll bring on a memory stick (.wav format) and play on Monday.

I’ve not heard from Moki or Randy but hope to before tomorrow morning.
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CLIP 1: BIOFILM GROWTH STAGES AND DISPERSAL. THIS SHOWS PLANKTONIC AND FRAGMENTING TYPES AND DIFFERENTIATES BETWEEN THE TWO PROCESSES AT THE END

YouTube – ‪Clip_One_Rough_Cut.mp4‬‎

Seconds: 00-12: (focus on spinal prosthetic)

Narration 1: To illustrate the stages of bacterial biofilm growth, we’ll show a patient with a prosthetic joint in their lumbar spine. The life cycle starts when bacteria enter the blood stream from any number of infection routes and evade the immune system or antibiotics. (18 seconds)

Seconds: 13-19: (showing pseudomonas entering blood stream):

Narration 2: Formation begins with the weak or reversible attachment of free-floating microorganisms to a surface. An organic layer composed of polysaccharides or glycoproteins adsorbs to the substrate, changing the physical properties of the host tissue or prosthetic surface. (18 seconds)

Seconds: 20-26: (bacteria “find” tissue to land on and then attach themselves):

Narration 3: If the host’s immune system or antibiotics do not clear the bacteria, they anchor themselves permanently using cell adhesion structures such as pili. This enables the arrival of other cells by providing diverse adhesion sites and build the matrix that holds the biofilm together. The biofilm is now encased in a protective matrix and becomes more resistant to host immunity.

Seconds 27-38: (bacteria creates a biofilm community)

Narration 4: Some species are not able to attach to a surface on their own and anchor themselves to the matrix or directly to earlier colonists. Cells now communicate via quorum sensing and the community grows through a combination of cell division and recruitment. The development of this complex, polymicrobial biofilm makes these communities increasingly antibiotic resistant and includes diverse species of bacteria’s, viruses, yeasts, minerals and more.

Seconds: 39- 49(split screen shows both types of dispersal: planktonic and fragmenting, zooming in on planktonic)

Narration 5: The biofilm also recruits excess neutrophils, pro-inflammatory cytokines and excessive host-derived proteases, providing it with nutrients. Bacteria now exist in multiple forms and metabolic states. The biofilm itself is now its own “phenotype” and becomes nearly impossible to eradicate. At a future date, it continue its life cycle by releasing entire fragments of the biofilm community and/or enabling planktonic bacteria to once again begin the infection process.