CDIABU Animation Lab

This topic contains 52 replies, has 4 voices, and was last updated by  Harrison 8 years, 5 months ago.

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  • #2905 Score: 0

    Harrison
    Keymaster
      2 pts

      This post is for the digital animation class. Post here with any comments or questions on the animations to be created for the film.

      This is a very helpful introductory to biofilms:

      Biofilm primer

      This is posted in the “Introduction to Biofilms” forum as well.

    • #3376 Score: 0

      Harrison
      Keymaster
        2 pts

        Here’s a link to the pseudomonas bacteria, which we will be modeling:

        Molecular Movies – A Portal to Cell and Molecular Animation

        Search for pseudo, then click on the animation….

        Pseudomonas | Graham Johnson

      • #3377 Score: 0

        Harrison
        Keymaster
          2 pts

          PDF attached. If you need the hyper links, I can send you the Word version.
          STAGES OF BIOFILM DEVELOPMENT.pdf

        • #3378 Score: 0

          Harrison
          Keymaster
            2 pts

            Dr. Randy Wolcott’s ground-breaking paper is attached. It’s the first paper that summarizes the depth and breadth of how chronic bacterial biofilm infections affect so many people (millions) and increase the costs of healthcare (billions). The table also describes the number of deaths, but does not provide a “suffering quotient” or “index” that describes how many people suffer with chronic infections.

            Not included in this paper:

            – 43,000,000 people with chronic sinusitis;
            – millions of people with unreported or undiagnosed chronic bacterial infections.
            Chronic wounds medical biofilm.pdf

          • #3379 Score: 0

            Harrison
            Keymaster
              2 pts

              Michael, Maria, Matthew, Nicholas, Michael, Sean, Randy, Andrew, Amanda, Kiran!

              Thanks for your time today. It was a very motivating and positive experience for me in so many ways. I realize I presented vast amounts of information, but I hope I was able to provide the high points that we’ll need to get started. To encourage proactive communications, please:

              1. Subscribe to this topic, so you will know when any of us post to it. You should be emailed when a new post is published (Thread tools > subscribe to this thread)

              2. Upload a picture as an avatar to your profile; use your headshot if you are amenable. See http://www.biofilmcommunity.org/profile.php?do=editprofilepic If you don’t want to use your own picture, choose your “favorite microbe!”

              3. Call or email anytime with questions!

              Thanks again.

            • #3380 Score: 0

              Harrison
              Keymaster
                2 pts

                Hi all,

                I just spoke to Dr. Wolcott, a biofilm expert and a probono consultant for this film project. He provided a helpful article which includes a nice graphic of a biofilm; pls see the attached: s.

                Please note:

                – This is very technical information. Feel free to read the article, but the main thing to focus on is the illustration on p. 1320, diagram of a medical biofilm.

                – Randy suggested the importance of showing the TWO WAYS in which a biofilm colony can “seed” other colonies elsewhere in the body. These two ways are:

                1. Seeding dispersal;
                2. Detachment fragments.

                I’ll discuss / reference these stages in another post.
                Biofilms cause persistent infections.pdf

              • #3381 Score: 0

                Harrison
                Keymaster
                  2 pts

                  I am hesitant to post this article because it is long and technical. Also, there’s limited amount of content that speaks directly to the final stages & dispersion of biofilm. Nonetheless, here it is.

                  😉
                  Stoodley Biofilms differentiated communities.pdf

                • #3382 Score: 0

                  Harrison
                  Keymaster
                    2 pts

                    Film 1: 20-40 seconds with Audio
                    Animation of the 5 stages of growth from seeding to formation of biofilm to planktonic shower. Focusing on formation developing on artificial surface such as hip joint or spinal disk.

                    Film 2: 20-40 seconds (possibly with Audio)
                    Animation following the planktonic shower dispersing into the blood, arrival of antibiotics killing all but .1%. zoom out then zoom back into another area of the body as that .1% as it reseeds .

                    (if time allows):

                    “Heavy slimers” bacterial bioilm growth, showing a much thicker layer of bioilm polymer around the bacteria.

                    “Intubation tube contamination” depicting insertions of intubation tube through the mouth, where it picks up microbes and carries them to the lungs where a new infection builds.

                  • #3383 Score: 0

                    Amanda
                    Member

                      We can do both options of the final stage, the only hesitation we have is the continuity of the the second film. It would be a different animation for film, from what we’ve read and been told (and I could very well be wrong) Antibiotics can’t get through the film, and if they’ve broken off in a clump of biofilm it would just go straight to the “reseed”. If there were individual bacteria then we can keep the scene where the antibiotics kill off most of the bacteria but leave some to reseed.

                      What are your feelings?

                    • #3384 Score: 0

                      Amanda
                      Member

                        Firstly I’m sorry it says pseudomonIs not pseudomonas, I realized after putting them through it was misspelled, wont happen again.

                        There are three movies, anyone with access to this forum can view them but they’re not listed in the youtube directory.
                        film 1
                        YouTube – storyboard.avi

                        film 2- planktonic shower version
                        YouTube – storyboard2.avi

                        Flim 2- fragment detachment version
                        YouTube – storyboard3.avi

                        I’ll post some screen shots and renders of what we’ve come up with 3D wise later this afternoon.

                      • #3385 Score: 0

                        Amanda
                        Member

                          [ATTACH=CONFIG]31[/ATTACH][ATTACH=CONFIG]32[/ATTACH][ATTACH=CONFIG]33[/ATTACH][ATTACH=CONFIG]34[/ATTACH][ATTACH=CONFIG]35[/ATTACH]
                          pseudomonas cells.jpg
                          lungs.jpg
                          disk_prosthetics_06.jpg
                          Body_Spine_Crystal_test_Comp.jpg
                          KneeBones2.jpg

                        • #3386 Score: 0

                          Amanda
                          Member

                            apparently it’s only 5 at a time.[ATTACH=CONFIG]36[/ATTACH][ATTACH=CONFIG]37[/ATTACH][ATTACH=CONFIG]38[/ATTACH][ATTACH=CONFIG]39[/ATTACH][ATTACH=CONFIG]40[/ATTACH]

                            if there are inaccuracies please let us know!
                            veins wall.jpg
                            spine 2.jpg
                            red blood cell.jpg
                            pseudomonas cells biofilm.jpg
                            spine.jpg

                          • #3387 Score: 0

                            Harrison
                            Keymaster
                              2 pts

                              Outstanding efforts all! I looked at all the video animations and they look great, as do the pictures. I will view each several times and provide more specific feedback in the form of comments and questions. I’ll ask for feedback from others too!

                              Rich

                            • #3388 Score: 0

                              Harrison
                              Keymaster
                                2 pts

                                FILM 1: YOUTUBE – STORYBOARD.AVI

                                Some comments in order of time sequence in seconds:

                                1. 14 seconds: I am confused about the solitary bug on the surface, only in terms of the incoming pseudomonas;
                                2. 24 seconds: Is the first bug shown that’s grown into the new colony? The issue of growth and the connectedness must be easily grasped by any viewer;
                                3. 37 seconds: the side by side comparison is awesome! Can we zoom in on each of the two dispersal types, then show complete separation:

                                a. planktonic pseudomonas leaving the biofilm
                                b. biofilm mass with planktonic components separating the colony

                                Related comments:

                                1. I like the style, color and overall effects;
                                2. The pseudomonas should have a “wiggly” tail

                                FILM 2- PLANKTONIC SHOWER VERSION: YOUTUBE – STORYBOARD2.AVI

                                1. Why so many spelling errors? ; )
                                2. However pseudomonas is depicted in one video should be consistent for others.
                                3. I love the colors and contrast in this one; this helps viewers more easily differentiate between objects and helps with the intuitive aspects of understanding “spatial relationships,” a very important concept in this biology lesson which is very abstract for most people.
                                4. For this animation, let’s start with a chronic wound in a patient’s foot. Show detachment from a deep, ugly, infected foot (I have pictures and videos).
                                5. Excellent flow, design, logic

                                FILM 2- FRAGMENT DETACHMENT VERSION: YOUTUBE – STORYBOARD3.AVI

                                1. Can we start this one with another source of infection? See table 1 in Dr. Randy Wolcott’s article CHRONIC WOUNDS AND THE MEDICAL BIOFILM PARADIGM. A common infection involves catheters, so it would be educational, and synergize with the video content in the film.
                                2. Are we using this style of animation? (See previous comment.)

                                Other comments: Can we use all of the animations for the film?

                              • #3389 Score: 0

                                Moki
                                Member

                                  I’m really impressed with these storyboard/animatics! These are going to be great! I don’t want to put the cart before the horse, but is it possible to see the models of the actors in the piece? That would help solidify the look and feel before you get down and dirty with the animation.

                                  moki

                                • #3390 Score: 0

                                  Moki
                                  Member

                                    Sorry, my bad for not seeing the second page. The models look great! I agree with Rich that they should have squiggly tails. I’m curious how they are going to move? They seem somewhat stiff right now but that might be because they are just stills. Having them tumble around the way they actually would do in the blood stream might be a bit more realistic and give an organic feel to them.

                                    What do you think Rich? Is this important or am I getting caught up in the details top much?

                                    m

                                  • #3391 Score: 0

                                    Harrison
                                    Keymaster
                                      2 pts

                                      Moki, this is good information indeed. At this point, any feedback is useful for all of us. We are all breaking new ground with this animation and there be many assumptions and judgement calls along the way. I enjoy the challenges that this creation process presents.

                                      Team CDIBU, I have a few more “fun” challenges for you to consider:

                                      1. I’d like to name the project that describes all these animations. Let’s brainstorm today; we’ll decide in our call this morning. Should the team be named too!?

                                      2. I’d like you to come up with the image for a DVD which will contain all these animations. I’d like you all to have these for your own professional portfolio. Also, I’d like to include a video of me presenting our work for this project. I’ll use this DVD for various presentations in the coming months. At some point, I’d like to have a conversation about copyrighting this content too; as there will be a widespread interest in it for myriad reasons.

                                      Team “x,” talk to you shortly!

                                    • #3392 Score: 0

                                      Harrison
                                      Keymaster
                                        2 pts

                                        BTW, the animations that you are creating will be “news” to most of America, as will be the film to be released in Q1 of 2011. This film is a potential “game changer” in health care – it will help patients, doctors and researchers rethink how they deal with chronic health conditions. So, some creative themes to consider for the project, relative to the previous post:

                                        – Change;
                                        – Health care improvement;
                                        – Education;
                                        – New paradigms in thinking about disease;
                                        – Helping people by relieving suffering;
                                        – Saving tax dollars;
                                        – Listening to a new message, perhaps a “hail mary” pass;
                                        – The biology lesson that was skipped in 8th grade.

                                        Get it? Good. 😉

                                      • #3393 Score: 0

                                        Harrison
                                        Keymaster
                                          2 pts

                                          Nice job today, team x! One of my follow-up tasks is below.
                                          __________________________________________

                                          Foot wound video:

                                          YouTube – Foot Ulcer

                                          Wound image with vasculature:

                                          http://www.theholisticcare.com/cure%20diseases/Images/diabetes/diabetic_foot_ulcer.jpg

                                          Or review images by searching Google images, using the term:

                                          diabetic foot wound

                                          Or try this link and see if this works:

                                          diabetic foot wound

                                        • #3394 Score: 0

                                          Harrison
                                          Keymaster
                                            2 pts

                                            As we discussed earlier, I would like to embed the foundation’s corporate logo in each animation, since this video will be mass-viewed/embedded/lifted in the future. The logo is attached.

                                            Perhaps it could be inserted in this way, I’ll defer to the group to decide:

                                            1. Show in a corner of the frame for 2-3 seconds in the beginning or end of the clip
                                            2. Show only in the beginning or end;
                                            3. Show throughout.

                                            Whatever the case, it should be unobtrusive. If this file is not sufficient for whatever reason, I can email it to Amanda as a precautionary measure.
                                            rgb_apf.jpg

                                          • #3395 Score: 0

                                            Amanda
                                            Member

                                              Thanks for the feedback Richard!
                                              And thanks for the videos, those will come in handy. We’ll decide on how best to incorporate the icon in the video but one idea tossed out there was like they have the NBC peacock grayed out and visible in the corner throughout.

                                              Minutes from this morning’s meeting.

                                              Film one

                                                [*]We’ll be adding more (10-20) introductory bacteria, and the tails will be moving.
                                                [*]Final spinal implant to be decided today by Richard, in terms of most surface area in relation to bacteria growth.
                                                [*]Time/preliminary animation will be worked out by us Thursday this week to help aid narration length.
                                                [*]The final scene will be side by side comparison of the shower and the fragmenting zooming in close up as they detach.

                                              Film two

                                                [*]Sticking with the presented color scheme.
                                                [*]Planktonic shower will be the “hero” of the second film.
                                                It will start from a chronic diabetic foot ulcer from which we’ll zoom in to find the bacteria moving through the blood and begins the
                                                “chase scene”
                                                [*]Lands on knee prosthetic and begins the process again.

                                              Film Three to be done if there is time, or after practicum is completed, would involve a detachment fragment and start from a catheter.

                                              Duration of clips will fall to us, but must be fixed, in terms of adding more animation, by the time final renders start on the 13th.

                                              We will get preliminary animations for the first two clips for view by the 5th, Richard will get us a preliminary script by Monday the 9th and which disk we should be using for the animation.

                                              Thanks again!

                                            • #3396 Score: 0

                                              Harrison
                                              Keymaster
                                                2 pts

                                                I’ll read it more carefully later today and may reply with more detail. In the meantime, going forward, please use this artificial disc image (it’s a Synthes ProDisc):

                                                lumber+fake+disk.jpg (image)

                                                Or try this link with Google Images:

                                                ADR Images

                                                The few infections I’ve seen first-hand in patients involve this device…

                                              • #3397 Score: 0

                                                Amanda
                                                Member

                                                  We decided on “bio-lumination” and we’re the Bio-luminaries
                                                  since we’re helping to illuminate people to the biofilm subject. Nod to Sean!
                                                  Sound good?

                                                • #3398 Score: 0

                                                  Harrison
                                                  Keymaster
                                                    2 pts

                                                    You guys are one-stop shopping!

                                                    😉

                                                  • #3399 Score: 0

                                                    Amanda
                                                    Member

                                                      Film One:
                                                      YouTube – ‪Clip_One_Rough_Cut.mp4‬‎

                                                      Film Two:
                                                      YouTube – ‪Clip_Two_Rough_Cut.mp4‬‎

                                                      View with a grain of salt till I can add some commentary a little later, but first I must eat! 😮

                                                      Amanda

                                                    • #3400 Score: 0

                                                      Harrison
                                                      Keymaster
                                                        2 pts

                                                        This is amazing work. Wait a minute, you guys are in a class and did this?! 🙂

                                                        I watched them both and they look great. I’ll watch them 1.1 zillion times later today and provide detailed feedback tonight/tomorrow.

                                                        I will also ask for input from Randy and Moki. Also, I will start with the scripts; Randy’s help is key with these…

                                                        Outstanding work! 😀

                                                      • #3401 Score: 0

                                                        Amanda
                                                        Member

                                                          We’re so psyched you like them!

                                                          Here are a few notes to keep in mind everyone while watching them,

                                                          Film one:
                                                          Is not to scale yet, its just at 640 for right now.
                                                          Please ignore the bump in the camera in the growth stage, that’s a simple adjustment we need to make in our editors.
                                                          The camera pan around the growth is going to be adjusted.
                                                          The split screen at the end will hold a few frames longer and can hold longer than that if you need more time in the script.
                                                          Also the Fragment dispersal is not the final design, but just so we could get the comparison for right now.
                                                          This is not the final production quality, 😮 can’t stress that enough! There’s still some calculating and tweaking, the growth is automated and adjustments take time.

                                                          Question regarding the introduction, we had an idea to start with your logo and the head becomes the crystal ball. This would be good for the stand alone but we’d make it so it could be cut out for the insertion into the movie. Does that sound interesting?

                                                          Film 2
                                                          We wanted to add more of a transition between the shower and the start of the chase scene just to give better context. This will add a extra 5 seconds or so.

                                                          Lastly do you like the icon in the corner the whole time? We can leave it out for film insertion but add them for the stand alone internet versions.

                                                          OK, I think that’s what all I have. Watch, enjoy, and thanks again! And have a wonderful weekend!

                                                          Bio-luminaries

                                                          .. see, spell check!

                                                        • #3402 Score: 0

                                                          Harrison
                                                          Keymaster
                                                            2 pts

                                                            Hey all,

                                                            I viewed the videos in detail and will provide comments in person tomorrow. (Amanda, can we do 1:00 PM instead of 11:00 AM?).

                                                            I am working on the first draft of the first video, editing it down while making the audio file, which I’ll bring on a memory stick (.wav format) and play on Monday.

                                                            I’ve not heard from Moki or Randy but hope to before tomorrow morning.
                                                            _____________________________

                                                            CLIP 1: BIOFILM GROWTH STAGES AND DISPERSAL. THIS SHOWS PLANKTONIC AND FRAGMENTING TYPES AND DIFFERENTIATES BETWEEN THE TWO PROCESSES AT THE END

                                                            YouTube – ‪Clip_One_Rough_Cut.mp4‬‎

                                                            Seconds: 00-12: (focus on spinal prosthetic)

                                                            Narration 1: To illustrate the stages of bacterial biofilm growth, we’ll show a patient with a prosthetic joint in their lumbar spine. The life cycle starts when bacteria enter the blood stream from any number of infection routes and evade the immune system or antibiotics. (18 seconds)

                                                            Seconds: 13-19: (showing pseudomonas entering blood stream):

                                                            Narration 2: Formation begins with the weak or reversible attachment of free-floating microorganisms to a surface. An organic layer composed of polysaccharides or glycoproteins adsorbs to the substrate, changing the physical properties of the host tissue or prosthetic surface. (18 seconds)

                                                            Seconds: 20-26: (bacteria “find” tissue to land on and then attach themselves):

                                                            Narration 3: If the host’s immune system or antibiotics do not clear the bacteria, they anchor themselves permanently using cell adhesion structures such as pili. This enables the arrival of other cells by providing diverse adhesion sites and build the matrix that holds the biofilm together. The biofilm is now encased in a protective matrix and becomes more resistant to host immunity.

                                                            Seconds 27-38: (bacteria creates a biofilm community)

                                                            Narration 4: Some species are not able to attach to a surface on their own and anchor themselves to the matrix or directly to earlier colonists. Cells now communicate via quorum sensing and the community grows through a combination of cell division and recruitment. The development of this complex, polymicrobial biofilm makes these communities increasingly antibiotic resistant and includes diverse species of bacteria’s, viruses, yeasts, minerals and more.

                                                            Seconds: 39- 49(split screen shows both types of dispersal: planktonic and fragmenting, zooming in on planktonic)

                                                            Narration 5: The biofilm also recruits excess neutrophils, pro-inflammatory cytokines and excessive host-derived proteases, providing it with nutrients. Bacteria now exist in multiple forms and metabolic states. The biofilm itself is now its own “phenotype” and becomes nearly impossible to eradicate. At a future date, it continue its life cycle by releasing entire fragments of the biofilm community and/or enabling planktonic bacteria to once again begin the infection process.

                                                          • #3403 Score: 0

                                                            Amanda
                                                            Member

                                                              Hello Richard!
                                                              Looks good so far!
                                                              1 p.m. should be fine, we have a meeting with our advisor at 1:30 but it’s so she can get a progress report. I’m seeing if she can come earlier but even if she can’t, if you don’t mind a little extra company.. ?

                                                              See you tomorrow!

                                                            • #3404 Score: 0

                                                              Harrison
                                                              Keymaster
                                                                2 pts

                                                                More comments & posts coming tonight. Here are some nice images for this week or new animations at a future date.
                                                                WOUND BIOFILM.jpg
                                                                Biofilm Drawing.jpg
                                                                Wound-biofilm-figure-A.doc

                                                              • #3405 Score: 0

                                                                Harrison
                                                                Keymaster
                                                                  2 pts

                                                                  CLIP 1: BIOFILM GROWTH STAGES AND DISPERSAL. THIS SHOWS PLANKTONIC AND FRAGMENTING TYPES AND DIFFERENTIATES BETWEEN THE TWO PROCESSES AT THE END

                                                                  YouTube – ‪Clip_One_Rough_Cut.mp4‬‎

                                                                  Seconds: 00-12: (focus on spinal prosthetic)

                                                                  Narration 1: To illustrate the stages of bacterial biofilm growth, we’ll show a patient with a prosthetic joint in their lumbar spine. The life cycle starts when bacteria enter the blood stream from any number of infection routes and evade the immune system or antibiotics. (18 seconds)

                                                                  Seconds: 13-19: (showing pseudomonas entering blood stream):

                                                                  Narration 2: Formation begins with reversible attachment of planktonic bacteria to a surface. A layer composed of polysaccharides or glycoproteins adsorbs into the substrate, changing the physical properties of the surface. Created by the human host, this conditioning coat now on the prosthetic surface is composed of fibrinogen, intravascular proteins and immune cells. Irreversible attachment to these host proteins is a strong signal to the bacterium to begin biofilm formation and is the first committed step of biofilm growth. (34 seconds)

                                                                  Seconds: 20-26: (bacteria “find” tissue to land on and then attach themselves):

                                                                  Narration 3: If the host’s immune system or antibiotics do not clear the bacteria, they anchor themselves more permanently using cell adhesion structures such as pili and receptors called epitopes. This encourages new species of bacteria to attach to newly arriving cells providing diverse adhesion sites and build the matrix that holds the biofilm together. The biofilm are now encased in a protective matrix and become more resistant to host immunity. (29 seconds)

                                                                  Seconds 27-38: (bacteria creates a biofilm community)

                                                                  Narration 4: Some species are not able to attach to a surface and anchor themselves to the matrix or directly to earlier colonists. Cells are now able to communicate via quorum sensing and use small molecules to regulate gene expression within the bacterium that make up the biofilm. The community grows through both cell division and recruitment. The development of this complex, polymicrobial biofilm makes these communities increasingly antibiotic resistant and includes diverse species of bacteria’s, yeasts, minerals and host proteins. (37 seconds)

                                                                  Seconds: 39- 49(split screen shows both types of dispersal: planktonic and fragmenting, zooming in on planktonic)

                                                                  Narration 5: The biofilm continues to recruit excess neutrophils, pro-inflammatory cytokines and host-derived proteases, producing a plasma exudate, a continuous source of nutrients. Bacteria now exist in multiple forms and metabolic states. The biofilm itself is now its own “phenotype” and becomes nearly impossible to eradicate. At some future date, it may continue its life cycle by releasing entire fragments of the biofilm community and/or enabling planktonic bacteria to once again begin the infection process. (39 seconds)

                                                                  Total length: 157 seconds 😮
                                                                  NARRATION SCRIPT ONE-Randy’s Comments.doc
                                                                  WOUND BIOFILM.jpg

                                                                • #3406 Score: 0

                                                                  Amanda
                                                                  Member

                                                                    I bold-ed thetime from our recordings.

                                                                    Narration 1: The life cycle starts when bacteria enter the blood stream from any number of infection routes and evade the immune system or antibiotics. (8 seconds)

                                                                    Narration 2: Formation begins with reversible attachment of planktonic bacteria to a surface. A layer composed of polysaccharides or glycoproteins adsorbs into the substrate, changing the physical properties of the surface. Created by the human host, this conditioning coat now on the prosthetic surface is composed of fibrinogen, intravascular proteins and immune cells. Irreversible attachment to these host proteins is a strong signal to the bacterium to begin biofilm formation and is the first committed step of biofilm growth. (30 seconds)

                                                                    Narration 3: If the host’s immune system or antibiotics do not clear the bacteria, they anchor themselves more permanently using cell adhesion structures such as pili and receptors called epitopes. This encourages new species of bacteria to attach to newly arriving cells providing diverse adhesion sites and build the matrix that holds the biofilm together. The biofilm are now encased in a protective matrix and become more resistant to host immunity. (25 seconds)

                                                                    Narration 4: Cells are now able to communicate via quorum sensing and use small molecules to regulate gene expression within the bacterium that make up the biofilm. The community grows through both cell division and recruitment. The development of this complex, polymicrobial biofilm makes these communities increasingly antibiotic resistant and includes diverse species of bacteria’s, yeasts, minerals and host proteins. (23 seconds)

                                                                    Narration 5: The biofilm continues to recruit excess neutrophils, pro-inflammatory cytokines and host-derived proteases, producing a plasma exudate, a continuous source of nutrients. Bacteria now exist in multiple forms and metabolic states. The biofilm itself is now its own “phenotype” and becomes nearly impossible to eradicate. At some future date, it may continue its life cycle by releasing entire fragments of the biofilm community and/or enabling planktonic bacteria to once again begin the infection process. (31 seconds)

                                                                    So just rehashing what we talked about, it needs to be 27 seconds less. Bringing the total time to 90 seconds.

                                                                    Thanks again for coming in today, it was very helpful!

                                                                  • #3407 Score: 0

                                                                    Amanda
                                                                    Member

                                                                      YouTube – ‪Clip_Two_Rough_Cut_3.mp4‬‎
                                                                      This is the version we showed you this afternoon, plan for total time 50 seconds.

                                                                    • #3408 Score: 0

                                                                      Harrison
                                                                      Keymaster
                                                                        2 pts

                                                                        Hi Amanda,

                                                                        Sorry, can we have a quick chat regarding this narration reconciliation? I am at 781-937-8843.

                                                                        : o

                                                                      • #3409 Score: 0

                                                                        Harrison
                                                                        Keymaster
                                                                          2 pts

                                                                          Coming in 10 minutes!

                                                                          ; )

                                                                        • #3410 Score: 0

                                                                          Harrison
                                                                          Keymaster
                                                                            2 pts

                                                                            This version might do it. Pls test it and let me know if it’s OK.

                                                                            😮
                                                                            Film 1 Narration.doc

                                                                          • #3411 Score: 0

                                                                            Harrison
                                                                            Keymaster
                                                                              2 pts

                                                                              This is a rough draft….less detailed than film 1, but that’s OK!
                                                                              _______________________

                                                                              YouTube – ‪Clip_Two_Rough_Cut.mp4‬‎

                                                                              Seconds: 00-5: (focus on foot wound)

                                                                              Narration: Diabetic foot wounds often involve bacterial biofilm infections.

                                                                              Seconds: 7-10 (zoom in on planktonic bacteria in the foot wound)

                                                                              Narration: Upon closer inspection, we see that an entrenched, mature biofilm community is releasing planktonic bacteria into the patient’s bloodstream.

                                                                              Seconds 11-20 (bacteria moving through the blood and begins the “chase scene”)

                                                                              Narration: The host immune system responds by sends a flurry of immune cells that attack the invaders. The majority of planktonic bacteria are killed by specialized immune cells, but some bacteria evade attack by using sophisticated tactics.

                                                                              Seconds 21-25: (bacteria escape immune system or antibiotics and find host tissue):

                                                                              Narration: Bacteria that escape find a suitable surface on which to settle and start the entire growth process over again.

                                                                              Seconds 26-33 (bacteria land on knee prosthetic and begins the process again):

                                                                              Narration: Unfortunately, a bacterial biofilm community has now colonized on a prosthetic knee implant and may present problems to the patient.

                                                                            • #3412 Score: 0

                                                                              Amanda
                                                                              Member

                                                                                Thank you for getting those to us so quickly, we timed them out and the first comes to 95seconds but it’s fine we’ll work with it. The second times out perfectly! Thank you!

                                                                                More to follow as the week goes on!

                                                                                Amanda

                                                                              • #3413 Score: 0

                                                                                Amanda
                                                                                Member

                                                                                  Here is a snipit for you, my classmate Matt will post one a little later as well but just so you can start to see how it will look.
                                                                                  There is one more effect we’re adding to make it look like a liquid environment.

                                                                                  This is from clip two the second to last scene after the chase, where they touch down on the knee surface.
                                                                                  YouTube – 2nd Animation Reseed

                                                                                  Have a good weekend!
                                                                                  Amanda

                                                                                • #3414 Score: 0

                                                                                  Harrison
                                                                                  Keymaster
                                                                                    2 pts

                                                                                    Outstanding work!

                                                                                    😀

                                                                                  • #3415 Score: 0

                                                                                    MattMcCarthy
                                                                                    Member

                                                                                      Here is the chase scene clip I’ve been working on…

                                                                                      YouTube – Chase Scene Through Blood

                                                                                    • #3416 Score: 0

                                                                                      Harrison
                                                                                      Keymaster
                                                                                        2 pts

                                                                                        Matt,

                                                                                        Well done. But a little too well done! I got motion sickness watching it! 😉

                                                                                        Just kidding. It will be interesting to see the end product; its speed, synchronicity with the narration, etc.

                                                                                        I had the thought if we should anywhere insert text that says something like “oversimplified for animation purposes…”

                                                                                        Any way, see you Monday at 1:30 PM. Thanks!

                                                                                        Rich

                                                                                      • #3417 Score: 0

                                                                                        Harrison
                                                                                        Keymaster
                                                                                          2 pts

                                                                                          Hey Team,

                                                                                          Rather than lunch, I was hoping we could arrange the following schedule – though it will cut into your weekend (while helping you avoid rush hour traffic!):

                                                                                          4:00 PM: Pictures by the waterfall & bridge (there are steps there), appr. at 191 Moody Street on GoogleMaps.

                                                                                          4:30-7:30 PM: Early dinner at Biago’s Ristorante & Bar to celebrate the animations’ completion. It will be my treat to a dinner buffet, just a small way of saying, “Thank you for providing $25,000 worth of animation services!”

                                                                                          😀

                                                                                        • #3418 Score: 0

                                                                                          Harrison
                                                                                          Keymaster
                                                                                            2 pts

                                                                                            Hi everyone,

                                                                                            Sorry Friday did not work out for everyone. These are the revised plans:

                                                                                            1. We will have dinner next door at Ponzu on Tuesday afternoon. I’ve arranged some yummy menu items with Bill, the owner and chef. We’ll have some tasty dishes in the back room from 4:30 PM on to appr. 7:00 PM.

                                                                                            2. Your presentation is at 1:30 PM on Tuesday, but I’d like to precede yours with a brief informal overview & commentary for the other BU people I’ve not met. Can you all help me with this –even if it’s a guess:

                                                                                            – total animation time for BOTH videos;
                                                                                            – total amount of man hours for the entire project.

                                                                                            Can someone reply to let me know that the whole class get this message. Thanks again!!!

                                                                                            😎

                                                                                          • #3419 Score: 0

                                                                                            Amanda
                                                                                            Member

                                                                                              Hi there!
                                                                                              Ponzu sounds terrific, thank you so much!!

                                                                                              For your debriefing (rough estimate)
                                                                                              around 120 hours of animation
                                                                                              1440 total time ( lighting, texturing, modeling, post, storyboard etc) Phew!

                                                                                              What a month!

                                                                                              See you Tuesday and thank you again for the opportunity, you’re going to be floored!

                                                                                            • #3420 Score: 0

                                                                                              Harrison
                                                                                              Keymaster
                                                                                                2 pts

                                                                                                Here’s the menu for our Tuesday celebratory dinner:

                                                                                                Appetizers

                                                                                                Roti Prata (Stuffed Indian Bread)
                                                                                                Steamed Vegetable Dumplings
                                                                                                Wasabi Pork Dumplings

                                                                                                Entries

                                                                                                Vegetables with Red Coconut Curry
                                                                                                Tropical Mango Chicken
                                                                                                Baby Buck Choy
                                                                                                Malaysian Indian Style Noodle with Stir-Fried Vegetables

                                                                                                Coconut Rice
                                                                                                Brown Rice

                                                                                                Beverages will be served, but if you want wine or beer, there’s a cash bar there. Let me know if you have any other preferences or dietary restrictions and I’ll be happy to plan accordingly.

                                                                                              • #3421 Score: 0

                                                                                                Harrison
                                                                                                Keymaster
                                                                                                  2 pts

                                                                                                  Hey Mike et al,

                                                                                                  If you have the artwork image for the DVD cover, can you send it to me, or attach it here? Thx!

                                                                                                  😉

                                                                                                • #3422 Score: 0

                                                                                                  Amanda
                                                                                                  Member

                                                                                                    [ATTACH=CONFIG]48[/ATTACH] Hopefully this will translate for you, we can also throw it on the hard copy DVD for you!
                                                                                                    Have a good weekend!!!
                                                                                                    dvd cover2.jpg

                                                                                                  • #3424 Score: 0

                                                                                                    Harrison
                                                                                                    Keymaster
                                                                                                      2 pts

                                                                                                      Thanks everyone, for allowing me to be your first client. Yesterday was really exciting for me for a long list of reasons – here’s but a few of them:

                                                                                                      1. I couldn’t wait to see the animations, but I am glad I did! Like I said yesterday: WOW?! You all did a great job and should be very proud of your accomplishments! In the coming months, you will see how this project will help many people better understand the threat of biofilm infections.

                                                                                                      2. Giving me the chance to share and present my passion for this project was helpful and cathartic for me. Having one foot in the grave for several years because of the mycoplasma pneumonia infection, healing, then coming back to help others is really a testament of human spirit. Working as a team, we will make a difference with this film. Don’t EVER forget the VALUE and POWER of what you have done to help people!

                                                                                                      I had fun working with you as a group, and I am a bit sad that it has come to an end. I’ll continue to update this topic as I develop the animations further for YouTube, along with any other big announcements.

                                                                                                      I attached a low-res picture of the BioLuminares team herein. I have other pics that are 1.7 to 3 MB and “PhotoShop Worthy” if anyone cares to tweak them. ; )

                                                                                                      Thanks again everyone. Continue to do great things!
                                                                                                      BU Animation Class 010 (Large).jpg

                                                                                                    • #3425 Score: 0

                                                                                                      Harrison
                                                                                                      Keymaster
                                                                                                        2 pts

                                                                                                        Here’s one more pic. If anyone can tweak this, I’d love a copy to send to Dr. Wolcott, and maybe include in the DVD too. If anyone is amenable, I’ll send the high res copy.

                                                                                                        😀
                                                                                                        BU Animation Class 2 (Large).jpg

                                                                                                      • #3426 Score: 0

                                                                                                        Harrison
                                                                                                        Keymaster
                                                                                                          2 pts

                                                                                                          By now, everyone has decompressed and is working 28 hours a day, right?! ; )

                                                                                                          I just wanted to let you know that I’ve transferred/duped the animations to DVDs, so each of you can have your own copies. The label is quite unsexy; but the digital duplicator (Coptech Digital) can do some amazing color labels if we care to get fancy-schmanzy in the future.

                                                                                                          So, in the meantime, I’ll provide 10 copies to Kiran to make them available to you for your portfolio. As you may know, the files are hefty and will have to be transferred to the PC/Mac for playing (as far as I know). They are on a dual-layer DVD.

                                                                                                          Kiran, let’s touch base to arrange a drop-off or lunch….

                                                                                                          Also, I am networking a bit for you all in case it helps nurture some job opps for any of you.

                                                                                                          Thx again for the great work!!! 😎

                                                                                                        • #3427 Score: 0

                                                                                                          Harrison
                                                                                                          Keymaster
                                                                                                            2 pts

                                                                                                            Hey BioLuminators!

                                                                                                            I wanted to share some work in progress – it’s a 12 minute video / 3 part clip explaining the film Seventeen Million. Some of your creative genius is in here:

                                                                                                            YouTube – Pre-trailer for “Seventeen Million,” Documentary on Biofilms

                                                                                                            My presentation was put together quickly (my choice). Accordingly, I will be replaced with an actress, Alexi Panos, who will present on my behalf. We’ll dress her up a bit, but she already has done some high profile gigs, like with JetBlue.

                                                                                                            We will edit Alexi’s presentation for brevity and also shrink the PowerPoint bullets into more accessible bytes. If you have any feedback, we would love to hear it – on the YouTube site or by email or phone, whatever suits you.

                                                                                                            Your work is very much appreciated — I’ve not forgotten the value of what you created. And thank you Sean, for your extra efforts in getting this video put together and published!

                                                                                                            Thanks, merci beaucoup…hope all of you are well.

                                                                                                            🙂

                                                                                                          • #2906 Score: 0

                                                                                                            Harrison
                                                                                                            Keymaster
                                                                                                              2 pts

                                                                                                              I hope all of you are well! I just wanted to make sure you received copies of the animations you produced — which I transferred to DVD with my non-profit logo and such. Did everyone get a copy from Urvi?

                                                                                                              Thx again!

                                                                                                              Richard

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