Reply To: CDIABU Animation Lab

#3406 Score: 0

    I bold-ed thetime from our recordings.

    Narration 1: The life cycle starts when bacteria enter the blood stream from any number of infection routes and evade the immune system or antibiotics. (8 seconds)

    Narration 2: Formation begins with reversible attachment of planktonic bacteria to a surface. A layer composed of polysaccharides or glycoproteins adsorbs into the substrate, changing the physical properties of the surface. Created by the human host, this conditioning coat now on the prosthetic surface is composed of fibrinogen, intravascular proteins and immune cells. Irreversible attachment to these host proteins is a strong signal to the bacterium to begin biofilm formation and is the first committed step of biofilm growth. (30 seconds)

    Narration 3: If the host’s immune system or antibiotics do not clear the bacteria, they anchor themselves more permanently using cell adhesion structures such as pili and receptors called epitopes. This encourages new species of bacteria to attach to newly arriving cells providing diverse adhesion sites and build the matrix that holds the biofilm together. The biofilm are now encased in a protective matrix and become more resistant to host immunity. (25 seconds)

    Narration 4: Cells are now able to communicate via quorum sensing and use small molecules to regulate gene expression within the bacterium that make up the biofilm. The community grows through both cell division and recruitment. The development of this complex, polymicrobial biofilm makes these communities increasingly antibiotic resistant and includes diverse species of bacteria’s, yeasts, minerals and host proteins. (23 seconds)

    Narration 5: The biofilm continues to recruit excess neutrophils, pro-inflammatory cytokines and host-derived proteases, producing a plasma exudate, a continuous source of nutrients. Bacteria now exist in multiple forms and metabolic states. The biofilm itself is now its own “phenotype” and becomes nearly impossible to eradicate. At some future date, it may continue its life cycle by releasing entire fragments of the biofilm community and/or enabling planktonic bacteria to once again begin the infection process. (31 seconds)

    So just rehashing what we talked about, it needs to be 27 seconds less. Bringing the total time to 90 seconds.

    Thanks again for coming in today, it was very helpful!