February 9, 2011 at 4:27 am #2986HarrisonKeymaster2 pts
J Aquat Anim Health. 2010 Jun;22(2):95-101.
Potential for largemouth bass virus to associate with and gain protection from bacterial biofilms.
Nath S, Aron GM, Southard GM, McLean RJ.
Department of Biology, Texas State University-San Marcos, 601 University Drive, San Marcos, Texas 78666, USA.
Quantitative PCR (qPCR) was used to investigate whether largemouth bass virus (LMBV) can exist within biofilms. Suspended LMBV was partitioned into either laboratory-grown Pseudomonas fluorescens biofilms or pond-grown, mixed-population biofilms.
retained infectivity when tested on epithelioma papillosum cyprini tissue culture cells. The LMBV associated with P. fluorescens by sodium hypochlorite and an iodine-based compound (betadine) but were susceptible to ethanol.
Largemouth bass virus was not detected in biofilms or water from ponds that had previously contained LMBV-positive fish, suggesting either that the viral concentrations were below the detection limit of qPCR or that the fish represented the main LMBV reservoir.
This study illustrates the potential for LMBV to associate with bacterial biofilms and thereby gain protection from some chemical disinfectants.
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