December 3, 2010 at 5:21 pm #2944
A few months ago, I called Now Foods to inquire about probiotics and ended up having an interesting talk with one of their R & D people about the oral ecosystem. Attached, pls see one of their products that uses strep salivarius as a microbe to colonize and balance the flora in the mouth.
During my video interviews, I’ve learned about transmutation, so I am a little nervous about the possibilities of gene transference that could occur with pathogenic strains of strep. Other than this (irrational?) fear, this seems like a neat idea. In the meantime, I focus on natural and simple methods of oral hygiene: brushing, water pic, and pH balancing with natural foods.
In the meantime, I am reminded of an interview I had last month with Dr. Nicolas Loebel from Ondine Biomedical for the future film. This excerpt (from another of his interviews) provides more context and clarity that leads me to my concern:
March 22, 2011 at 4:58 pm #3449
American Society for Microbiology
Tips from the journals of the American Society for Microbiology
Streptococcus Enzyme Could Compete With Toothbrushes, Dental Floss
Investigators from Japan show in vitro that the bacterium Streptococcus salivarius, a non-biofilm forming, and otherwise harmless inhabitant of the human mouth, actually inhibits the formation of dental biofilms, otherwise known as plaque. Two enzymes this bacteria produces are responsible for this inhibition. The research is published in the March 2011 issue of the journal Applied and Environmental Microbiology.
“FruA may be useful for prevention of dental caries,” corresponding author Hidenobu Senpuku, of the National Institute of Infectious Diseases, Tokyo says of one of the enzymes. “The activity of the inhibitors was elevated in the presence of sucrose, and the inhibitory effects were dependent on the sucrose concentration in the biofilm formation assay medium,” the researchers write.
“We show that FruA produced by S. salivarius inhibited S. mutans biofilm formation completely in the in vitro assay supplemented with sucrose,” the researchers write. S. salivarius is the primary species of bacteria inhabiting the mouth, according to the report.
The authors suggest that FruA may actually regulate microbial pathogenicity in the oral cavity. They found that a commercial FruA, produced by Aspergillus niger, was as effective as S. salivarius FruA at inhibiting S. mutans biofilm formation, despite the fact that its amino acid composition is somewhat different from that of S. salivarius.
FruA is produced not only by S. salivarius, but by other oral streptococci. Much of the oral microbial flora consists of many beneficial species of bacteria that help maintain oral health and control the progression of oral disease.
(A. Ogawa, S. Furukawa, S. Fujita, J. Mitobe, T. Kawarai, N. Narisawa, T. Sekizuka, M. Kuroda, K. Ochiai, H. Ogihara, S. Kosono, S. Yoneda, H. Watanabe, Y. Morinaga, H. Uematsu, and H. Senpuku, 2011. Inhibition of Streptococcus mutans biofilm formation by Streptococcus salivarius FruA. Appl. Environ. Microbiol. 77:1572-1580.)
Contact: Jim Sliwa
June 11, 2011 at 11:42 pm #3456
From Oragenics, using these bacterial strains:
600 Million CFU Probiora3®
Streptococcus uberis KJ2
Streptococcus oralis KJ3
Streptococcus rattus JH145
Oragenics’ Oral Care Products EvoraPlus: A probiotic mint with equal portions of all three strains that is optimally designed for those over the age of 11. Taken twice daily (ideally after brushing) this probiotic mint supports gum and tooth health, naturally freshens breath and gently whitens teeth.
EvoraPro: A professional-strength version of EvoraPlus used to accelerate the repopulation of the beneficial bacteria that are removed along with bad bacteria during a professional prophylaxis. This highly concentrated blend of ProBiora3 probiotics comes in a mint form and is distributed exclusively by the dental professionals.
EvoraKids: A Wild Very Berry Cherry chew that has higher levels of JH145, which supports dental health, and reduced levels of KJ3 and KJ2, since periodontal health is not typically a concern for children.
Teddys Pride: An odorless and tasteless probiotic powder that has higher levels of KJ3 and KJ2, which address the tooth staining and breath problems common to dogs and cats, and reduced levels of JH145, since tooth decay is not typically a concern in companion pets.
In addition to these products, we also market ProBiora3 as an active ingredient for private label products and in bulk for licensing applications.
August 3, 2011 at 7:54 pm #2945
Influence of Streptococcus mutans on Enterococcus faecalis Biofilm Formation
Dong Mei Deng, PhD, Michel A. Hoogenkamp, BSc, Rob A.M. Exterkate, BSc, Lei Meng Jiang, DMD, Lucas W.M. van der Sluis, PhD, Jacob M. ten Cate, PhD, Wim Crielaard, PhD
An important virulence factor of Enterococcus faecalis is its ability to form biofilms. Most studies on biofilm formation have been carried out by using E. faecalis monocultures. Given the polymicrobial nature of root canal infections, it is important to understand biofilm formation of E. faecalis in the presence of other microorganisms.
Eight clinical strains of E. faecalis were tested for biofilm formation on hydroxyapatite disks in the presence and absence of a Streptococcus mutans biofilm.
Significantly more E. faecalis viable cells were found in biofilms in the presence of S. mutans. This phenomenon was, however, strain-dependent. Of the 8 strains tested, biofilm formation of strains AA-OR34, ER5/1, and V583 was not influenced by S. mutans biofilms.
The results from this study, especially the strain difference, underline the importance of studying biofilm formation in a more realistic multispecies setting.
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